Rapid Antigen Written Assessment

Children's Hospital of Phildadelphia Clinical Virology Laboratory Rapid Antigen Annual Written Assessment **IMPORTANT** Please enter your name and date in the comment box when you have completed the quiz.**

published on March 14, 2014
1/9

By checking the following boxes, you are verifying that you have:

Read and reviewed the Immunofluorescence Assay Standard Operating Procedures.
Read and reviewed the FA QC and Change of Lot form binder.
Read and reviewed the Rapids Package Insert binder.
2/9

Our laboratory method for detecting measles and/or mumps antigen is what kind of immunofluroescence assay?

DIRECT
INDIRECT
3/9

PREFERRED specimens for measles virus rapid antigen detection are:

Hint: 2 choices
Conjunctival swabs
Buccal swabs
Nasopharyngeal aspirates
Skin lesion scrapings
4/9

PREFERRED specimens for mumps virus rapid antigen detection are:

Hint: 2 choices
Conjunctival swabs
Buccal swabs
Nasopharyngeal aspirates
Skin lesion scrapings
5/9

What must be done to the double-well microscope slide prior to cytofunnel assembly?

Slide must be precleaned with 10% bleach solution
Slide must be precleaned with 95% ethanol.
Slide must be precleaned with 70% ethanol.
Slide must be rinsed thoroughly with distilled water.
6/9

Prior to evaluating a patient slide for positivity, immunofluorescence slides should first be evaluated for:

Hint: 3 choices
Cellular morphology
Cellular intactness
Sufficient number of columnar epithelial cells
Sufficient number of squamous epithelial cells
7/9

For questions 7-10, refer to the graphic representation below.

For our laboratory measles IFA methodology, which component is bound to the slide (the triangle in the graphic)?

For questions 7-10, refer to the graphic representation below. For our laboratory measles IFA methodology, which component is bound to the slide (the triangle in the graphic)?
Patient cellular material containing measles antibody
Patient cellular material containing measles antigen
Measles-specific mouse monoclonal antibody
Fluorescein-labeled goat anti-mouse antibody
8/9

For our laboratory measles IFA methodology, which component is bound to the triangle?

Patient cellular material containing measles antibody
Patient cellular material containing measles antigen
Measles-specific mouse monoclonal antibody
Fluorescein-labeled goat anti-mouse antibody
9/9

For our laboratory measles IFA methodology, which component allows for visual detection of antigen-antibody complexes?

Patient cellular material containing measles antibody
Patient cellular material containing measles antigen
Measles-specific mouse monoclonal antibody
Fluorescein-labeled goat anti-mouse antibody